Switch on the automacs pro separator the instrument will automatically initialize. The macs minisampler, attached to the automacs pro separator, can hold one of three different macs cooling tube racks containing up to six samples. Identification of comigrating proteins on the fluorchem m system. Alfa laval separators manuals and spare parts catalogs in pdf. Apr 24, 2012 overview the automacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. If the separator has been delivered and installed by alfa laval as a part of a processing system, this manual should be. Consult the user manual before operating this instrument for the first time. Automated cell isolation automacs pro separator usa. Automacs pro separator magnetic cell sorter available from the lab world group. Method for discovery of peptide reagents using a commercial.
Please read the user manual of this instrument carefully and pay attention to all warnings and precautions. Buffers used for operating the automacs pro separator or the automacs separator should have a temperature of. Automacs pro separator magnetic cell sorter youtube. By combining an exceptionally compact design, a simple, userfriendly interface and the fast and easy cell separation protocols youve come to expect from stemcell technologies, roboseps fits easily into the workflow of any lab that needs the multisample processing capacity, speed, reliability and convenience of automated cell isolation. Alfa laval separators manuals and spare parts catalogs in. Warnings and precautions the guidelines in this section explain the potential risks associated with the. Maximal care has been taken by miltenyi biotec in the preparation of this user manual. Column substitutes for longterm storage are provided with the automacs pro separator starter kit. The automacs pro separator is designed for safe use when installed correctly, operated by trained personel operated in accordance with general safety practices and the instructions in this user manual. The cell separation is programmed using the touchscreen and intuitive screen menus. For manual labeling follow labeling instructions in the macs microbeads datasheet. The following warnings and cautions are provided to help you prevent injury to yourself or damage to the instrument.
Cell sorters core facility flow cytometry bmc lmu munich. The epfl flow cytometry core facility fccf provides stateoftheart instrumentation, technical expertise, theoretical and technical training, and cell sorting service. This chapter describes the safety instructions and site requirements for your automacs pro separator. Manual and the installation manual should be interpreted so as. Maintenance and troubleshooting for magnetic separator.
Columns do not have a top or bottom and do not require special orientation in the column holder. For details refer to the section describing the cell separation programs in the respective user manual. Easy to use automated magnetic cell sorting biocompare. Stanford automacs pro user guide 072211 starting up 1. We are a largescale manufacturer specializing in producing various mining machines including different types of sand and gravel equipment, milling equipment, mineral processing equipment and building materials equipment. If you want to use it inside the hood, do not forget to. A utomacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. Magnetic separation with the automacs pro separator or the automacs separator refer to the respective user manual for instructions on how to use the automacs pro separator or the automacs separator. Automacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple sam. Cells can be automatically isolated even directly from whole blood or bone marrow, which makes it ideal for multiple myeloma research or lineagespecific chimerism analysis. If you make the solutions they should be filtered before use use a 0. Aug 21, 2018 the automacs pro separator is a benchtop magnetic cell sorter that allows gentle sorting of more than ten million cells per second from a sample of up to 4.
Indicates a hazard situation, which if not avoided, could result in minor or moderate injury. Check that all bottles are filled with the appropriate solutions. If the separator has been delivered and installed by alfa laval as. Protocols section of the respective separator user manual. Designed for the selection of target cells in a fully automated, walkaway fashion. It is a computercontrolled device for the automated separation of magnetically labeled cells using macs technology.
Uantitative analysis of 3color fluorescent western blots with the fluorchem q. Mif inhibition reverts the gene expression profile of human melanoma cell lineinduced mdscs to normal monocytes. A xerxes oilwater separator is a highquality system for removing oil from water. When using the program posselwb, the whole blood sample will be diluted 3fold the starting volume. If in doubt contact your local sales representative or miltenyi biotec automacs pro specialist. Employing the macs technology, the automacs separator is designed for positive selection as well as depletion of magnetically labeled cells. Morrison 9102014 page 23 the automacs pro separator is a benchtop instrument for highspeed automated magnetic cell sorting of multiple samples under standardized conditions. Rinsing buffer pbs, 2mm edta pbs, 2mm edta or facsrinse. After each cell separation, a thorough washing procedure rinses the columns of the automacs pro separator.
Persons who have not read and understood the instruction manual of the separator. To view the current software version, from the software menu select. Mif inhibition reverts the gene expression profile of. Empty the waste bottle and add 100ml bleach to the bottom. Unless otherwise specifically indicated, miltenyi biotec products and services are for research use only and not for therapeutic or diagnostic use. Depletion with the automacs pro separator or the automacs separator refer to the respective user manual for instructions on how to use the automacs pro separator or the automacs separator.
Here, we have developed a methodology for bacterial cell sorting using the semiautomated automacs pro separator for the first time, and have produced a complete method for sorting of bacteria. The automacs pro separator employs stateoftheart technology. Automated cell isolation with the automacs pro separator provides fast and gentle isolation of virtually any cell type. The automacs pro separator is a benchtop automated magnetic cell sorter for the processing of multiple samples. Automacs user guide the automacs allows biomagnetic cell sorting of cells labelled with macs microbeads. This chapter describes the safety instructions and site. Automacs pro separator auto pro provided by the beijing biolaunching technologies co. Persons who have not been briefed on proper and correct operation. Content 5 content 5 content chapter 1 15 1 important information 15 1.
The components of an automacs pro separator starter kit. The automacs pro separator is equipped with reusable automacs columns. The automacs pro separator is a benchtop instrument for highspeed magnetic cell sorting of multiple samples. The macs products are sold by miltenyi biotec online catalogue at. After priming, the instrument will automatically display the status menu. Save time with fully automated multisample cell separation. The macs minisampler connects to the automacs pro separator and thus represents a part of the cell separation device. Once installed, the automacs columns become part of a closed fluidic system and can be used for up to 14 days or 100 separations within 14 days. Miltenyi biotec is a global provider of products and services that advance biomedical research and cellular therapy. Program choice depends on the isolation strategy, the strength of magnetic labeling, and the frequency of magnetically labeled cells.
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